annatto seed extract (alkali-processed norbixin, not acid precipitated)
annatto G
Identification
| Name | annatto seed extract (alkali-processed norbixin, not acid precipitated) |
| IUPAC | (2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-tetramethylicosa-2,4,6,8,10,12,14,16,18-nonaenedioic acid |
| CAS Number | 542-40-5 |
| EINECS | 208-810-8 |
| FDA UNII | Search |
| Molecular Formula | C24 H28 O4 |
| Molecular Weight | 380.48396000 |
Regulatory
| FDA/DG SANTE Petitions, Reviews, Notices | EU SANCO |
| JECFA Food Additive | Annatto Extract (Alkali-Processed Norbixin, not Acid Precipitated) |
| GSFA Codex | Annatto extracts, norbixin-based (160b(ii)) |
| DG SANTE Food Additives | annatto G |
Physical Properties
| Appearance | dark red-brown to red purple powder (est) |
| Assay | 15.00 to 100.00 |
| Food Chemicals Codex Listed | No |
| Boiling Point | 640.37 °C. @ 760.00 mm Hg (est) |
| Flash Point | 671.00 °F. TCC ( 355.10 °C. ) (est) |
| logP (o/w) | 5.532 (est) |
| Soluble in | alkaline water |
No sensory data available
Safety Information
| Oral/Parenteral Toxicity | Not determined |
| Dermal Toxicity | Not determined |
| Inhalation Toxicity | Not determined |
GHS Classification
['GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)', 'GHS Label elements, including precautionary statements']
Safety in Use
| Category | coloring agents |
| Recommendation for annatto seed extract (alkali-processed norbixin, not acid precipitated) usage levels up to | not for fragrance use. |
| Recommendation for annatto seed extract (alkali-processed norbixin, not acid precipitated) flavor usage levels up to | not for flavor use. |
No supplier data available
Potential Uses
Natural Occurrence
Synonyms
annatto G
bixa orellana seed extract (alkali-processed norbixin, not acid precipitated)
c.i. 75120 (alkali-processed norbixin, not acid precipitated)
ci 75120 (alkali-processed norbixin, not acid precipitated)
natural orange 4 (alkali-processed norbixin, not acid precipitated)
Info:
EVALUATION OF CERTAIN FOOD ADDITIVES AND CONTAMINANTS
PubMed:
Gum arabic and Fe²⺠synergistically improve the heat and acid stability of norbixin at pH 3.0-5.0.
PubMed:
Studies on interaction of norbixin with DNA: multispectroscopic and in silico analysis.
PubMed:
Short communication: norbixin and bixin partitioning in Cheddar cheese and whey.
PubMed:
Short communication: Development of a novel method for the extraction of norbixin from whey and its subsequent quantification via high performance liquid chromatography.
PubMed:
Inhibition of cyclophosphamide-induced oxidative stress in rat brain by polar and non-polar extracts of Annatto (Bixa orellana) seeds.
PubMed:
Probing the binding between norbixin and dairy proteins by spectroscopy methods.
PubMed:
Effect of bixin and norbixin on the expression of cytochrome P450 in HepG2 cell line.
PubMed:
A thirteen-week oral toxicity study of annatto extract (norbixin), a natural food color extracted from the seed coat of annatto (Bixa orellana L.), in Sprague-Dawley rats.
PubMed:
Prooxidant activity of norbixin in model of acute gastric ulcer induced by ethanol in rats.
PubMed:
Annatto prevents retinal degeneration induced by endoplasmic reticulum stress in vitro and in vivo.
PubMed:
Raman spectroscopic determination of norbixin and tartrazine in sugar.
PubMed:
Characteristics of activated carbon and carbon nanotubes as adsorbents to remove annatto (norbixin) in cheese whey.
PubMed:
Bixin and norbixin have opposite effects on glycemia, lipidemia, and oxidative stress in streptozotocin-induced diabetic rats.
PubMed:
Contact sensitizing potential of annatto extract and its two primary color components, cis-bixin and norbixin, in female BALB/c mice.
PubMed:
Bixin and norbixin protect against DNA-damage and alterations of redox status induced by methylmercury exposure in vivo.
PubMed:
Norbixin ingestion did not induce any detectable DNA breakage in liver and kidney but caused a considerable impairment in plasma glucose levels of rats and mice.
PubMed:
Digestive stability and transport of norbixin, a 24-carbon carotenoid, across monolayers of Caco-2 cells.
PubMed:
Effect of temperature and concentration on benzoyl peroxide bleaching efficacy and benzoic acid levels in whey protein concentrate.
PubMed:
Toxicity of the hyperglycaemic-inducing extract of the annatto (Bixa orellana) in the dog.
PubMed:
Induction of liver monooxygenases by annatto and bixin in female rats.
PubMed:
Extraction of an hyperglycaemic principle from the annatto (Bixa orellana), a medicinal plant in the West Indies.
PubMed:
Decolorization of Cheddar cheese whey by activated carbon.
PubMed:
Antigenotoxic and antimutagenic potential of an annatto pigment (norbixin) against oxidative stress.
PubMed:
Biochemical behaviour of norbixin during in vitro DNA damage induced by reactive oxygen species.
PubMed:
Time-of-flight secondary ion mass spectrometry and X-ray photoelectron spectroscopy analyses of Bixa orellana seeds.
PubMed:
Inhibitory effect of apocarotenoids on the activity of tyrosinase: Multi-spectroscopic and docking studies.
PubMed:
A synergistic effect of Cu(2+) and norbixin on DNA damage.
PubMed:
Bixin and norbixin in human plasma: determination and study of the absorption of a single dose of Annatto food color.
PubMed:
Generalized and rapid supramolecular solvent-based sample treatment for the determination of annatto in food.
PubMed:
Hypoglycaemic activity of Bixa orellana extract in the dog.
PubMed:
Cold enzymatic bleaching of fluid whey.
PubMed:
Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection.
PubMed:
[Detection of the annatto dye norbixin/bixin in cheese using derivative spectroscopy and high performance liquid chromatography (HPLC)].
PubMed:
Alternative bleaching methods for Cheddar cheese whey.
PubMed:
Absence of liver tumor promoting effects of annatto extract (norbixin), a natural carotenoid food color, in a medium-term liver carcinogenesis bioassay using male F344 rats.
PubMed:
High-performance liquid chromatographic separation of carminic acid, alpha- and beta-bixin, and alpha- and beta-norbixin, and the determination of carminic acid in foods.
PubMed:
The use of lactoperoxidase for the bleaching of fluid whey.
PubMed:
Effect of bleaching permeate from microfiltered skim milk on 80% serum protein concentrate.
PubMed:
Effect of temperature and bleaching agent on bleaching of liquid Cheddar whey.
PubMed:
The effect of microfiltration on color, flavor, and functionality of 80% whey protein concentrate.
PubMed:
Bixin activates PPARα and improves obesity-induced abnormalities of carbohydrate and lipid metabolism in mice.
PubMed:
Sensory and Functionality Differences of Whey Protein Isolate Bleached by Hydrogen or Benzoyl Peroxide.
PubMed:
Influence of bleaching on flavor of 34% whey protein concentrate and residual benzoic acid concentration in dried whey proteins.
PubMed:
Short communication: The influence of solids concentration and bleaching agent on bleaching efficacy and flavor of sweet whey powder.
PubMed:
Influence of storage, heat treatment, and solids composition on the bleaching of whey with hydrogen peroxide.
PubMed:
Development of carotenoid storage cells in Bixa orellana L. seed arils.
PubMed:
Characterizing endogenous and exogenous peroxidase activity for bleaching of fluid whey and retentate.
PubMed:
Evaluation of certain food additives and contaminants.
PubMed:
Determination of annatto in high-fat dairy products, margarine and hard candy by solvent extraction followed by high-performance liquid chromatography.
PubMed:
Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties.
PubMed:
Bixin regulates mRNA expression involved in adipogenesis and enhances insulin sensitivity in 3T3-L1 adipocytes through PPARgamma activation.
PubMed:
Bioautography and chemical characterization of antimicrobial compound(s) in commercial water-soluble annatto extracts.
PubMed:
The effect of starter culture and annatto on the flavor and functionality of whey protein concentrate.
PubMed:
Effect of bleaching whey on sensory and functional properties of 80% whey protein concentrate.
PubMed:
Method development and analysis of retail foods for annatto food colouring material.
PubMed:
The effect of bleaching agent on the flavor of liquid whey and whey protein concentrate.
PubMed:
Carotenoids and β-cyclodextrin inclusion complexes: Raman spectroscopy and theoretical investigation.
PubMed:
In vitro scavenging capacity of annatto seed extracts against reactive oxygen and nitrogen species.
PubMed:
Neuron-glial cell communication in the traumatic stress-induced immunomodulation.
PubMed:
Effect of natural pigments on the oxidative stability of sausages stored under refrigeration.
PubMed:
Evaluation of the clastogenicity and anticlastogenicity of the carotenoid bixin in human lymphocyte cultures.
PubMed:
Novel method for the determination of added annatto colour in extruded corn snack products.
PubMed:
Annatto constituent cis-bixin has selective antimyeloma effects mediated by oxidative stress and associated with inhibition of thioredoxin and thioredoxin reductase.
PubMed:
Invited review: Annatto usage and bleaching in dairy foods.
PubMed:
Reactive oxygen species mediate lethality induced by far-UV in Escherichia coli cells.
PubMed:
Biosynthesis of the food and cosmetic plant pigment bixin (annatto).
PubMed:
Analysis of annatto (Bixa orellana) food coloring formulations. 2. Determination of aromatic hydrocarbon thermal degradation products by gas chromatography.
PubMed:
Characterization of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection.
PubMed:
Partial replacement of nitrite by annatto as a colour additive in sausage.
PubMed:
Structural analysis of the synaptic protein neuroligin and its beta-neurexin complex: determinants for folding and cell adhesion.
PubMed:
Chitosan microspheres containing the natural urucum pigment.