protease from bacillus amyloliquefaciens

protease from bacillus amyloliquefaciens PubMed: Immobilization of proteases on chitosan for the development of films with anti-biofilm properties. PubMed: Molecular diversity and functional variability of environmental isolates of Bacillus species. PubMed: Field evaluation of the bacterial volatile derivative 3-pentanol in priming for induced resistance in pepper. PubMed: Biocontrol traits and antagonistic potential of Bacillus amyloliquefaciens strain NJZJSB3 against Sclerotinia sclerotiorum, a causal agent of canola stem rot. PubMed: Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco. PubMed: Trypsin inhibitor from edible mushroom Pleurotus floridanus active against proteases of microbial origin. PubMed: Purification and partial characterization of a chymotrypsin-like serine fibrinolytic enzyme from Bacillus amyloliquefaciens FCF-11 using corn husk as a novel substrate. PubMed: Enhanced control of cucumber wilt disease by Bacillus amyloliquefaciens SQR9 by altering the regulation of Its DegU phosphorylation. PubMed: Characteristics of miniature Cheddar-type cheese made by microbial rennet from Bacillus amyloliquefaciens: a comparison with commercial calf rennet. PubMed: Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere. PubMed: Characteristics of Streptomyces griseus biofilms in continuous flow tubular reactors. PubMed: In vitro and in vivo characterization of plant growth promoting Bacillus strains isolated from extreme environments of Eastern Algeria. PubMed: Production and characterization of keratinolytic protease from new wool-degrading Bacillus species isolated from Egyptian ecosystem. PubMed: Isolation and characterization of a thermophilic Bacillus sp. with protease activity isolated from hot spring of Tarabalo, Odisha, India. PubMed: Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens SYB-001. PubMed: Induction of apoptosis in human cancer cells by a Bacillus lipopeptide bacillomycin D. PubMed: Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic. PubMed: Characterization of a fibrinolytic enzyme secreted by Bacillus amyloliquefaciens CB1 and its gene cloning. PubMed: Statistical optimization of medium components for milk-clotting enzyme production by Bacillus amyloliquefaciens D4 using wheat Bran-an agro-industry waste. PubMed: Isolation and characterization of antagonistic Bacillus strains capable to degrade ethylenethiourea. PubMed: Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action. PubMed: Chitin extraction from shrimp shell waste using Bacillus bacteria. PubMed: Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding. PubMed: Isolation and molecular characterization of a novel strain of Bacillus with antifungal activity from the sorghum rhizosphere. PubMed: Characterization of mycolytic enzymes of Bacillus strains and their bio-protection role against Rhizoctonia solani in tomato. PubMed: Comment on Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis. PubMed: Characterization of deamidation of barstar using electrospray ionization quadrupole time-of-flight mass spectrometry, which stabilizes an equilibrium unfolding intermediate. PubMed: Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju. PubMed: Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ5-41 isolated from Meju. PubMed: [Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani]. PubMed: Extracellular production of cycloisomaltooligosaccharide glucanotransferase and cyclodextran by a protease-deficient Bacillus subtilis host-vector system. PubMed: Directed evolution improves the fibrinolytic activity of nattokinase from Bacillus natto. PubMed: Overproduction of Bacillus amyloliquefaciens extracellular glutamyl-endopeptidase as a result of ectopic multi-copy insertion of an efficiently-expressed mpr gene into the Bacillus subtilis chromosome. PubMed: Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis. PubMed: Cloning and expression of a bpr gene encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1. PubMed: Protein folding pathways and state transitions described by classical equations of motion of an elastic network model. PubMed: Calculations of the binding affinities of protein-protein complexes with the fast multipole method. PubMed: Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway. PubMed: Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and heterologous signal peptides. PubMed: Chicken feathers: a complex substrate for the co-production of alpha-amylase and proteases by B. licheniformis NH1. PubMed: Engineering an artificial zymogen by alternate frame protein folding. PubMed: Fibrinolytic serine protease isolation from Bacillus amyloliquefaciens An6 grown on Mirabilis jalapa tuber powders. PubMed: Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH51 isolated from cheonggukjang. PubMed: Structure of a switchable subtilisin complexed with a substrate and with the activator azide. PubMed: ATP-dependent proteases differ substantially in their ability to unfold globular proteins. PubMed: An antifungal protein from Bacillus amyloliquefaciens. PubMed: Exploring the molecular design of protein interaction sites with molecular dynamics simulations and free energy calculations. PubMed: Peptide synthesis using carbamoylmethyl esters as acyl donors mediated by Bacillus amyloliquefaciens protease. PubMed: Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes. PubMed: Head-to-tail cyclized cystine-knot peptides by a combined recombinant and chemical route of synthesis. PubMed: Cholesterol lowering mechanism of soybean protein hydrolysate. PubMed: In silico screening of mutational effects on enzyme-proteic inhibitor affinity: a docking-based approach. PubMed: Purification and characterization of a subtilisin-like proteinases secreted in the stationary growth phase of Bacillus amyloliquefaciens H2. PubMed: Multi-template approach to modeling engineered disulfide bonds. PubMed: A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes. PubMed: Effects of degU32(Hy), degQa and degR pleiotropic regulatory genes on the growth and protease fermentation of Bacillus subtilis Ki-2-132. PubMed: Characterization of a novel PepF-like oligopeptidase secreted by Bacillus amyloliquefaciens 23-7A. PubMed: Denatured proteins and early folding intermediates simulated in a reduced conformational space. PubMed: Gene expression and characteristics of a novel fibrinolytic enzyme (subtilisin DFE) in Escherichia coli. PubMed: Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of alpha-amylase gene from Bacillus amyloliquefaciens. PubMed: A fusion protein system for the recombinant production of short disulfide bond rich cystine knot peptides using barnase as a purification handle. PubMed: Comparative studies on trifluoroethanol (TFE) state of a thermophilic alpha-amylase and its mesophilic counterpart: limited proteolysis, conformational analysis, aggregation and reactivation of the enzymes. PubMed: Isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts. PubMed: Stability parameters for one-step mechanism of irreversible protein denaturation: a method based on nonlinear regression of calorimetric peaks with nonzero deltaCp. PubMed: Cloning and expression of a fibrinolytic enzyme (subtilisin DFE) gene from Bacillus amyloliquefaciens DC-4 in Bacillus subtilis. PubMed: Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis. PubMed: Purification and characterization of proteases from Bacillus amyloliquefaciens isolated from traditional soybean fermentation starter. PubMed: The X-ray crystal structure of pyrrolidone-carboxylate peptidase from hyperthermophilic archaea Pyrococcus horikoshii. PubMed: Lack of a robust unfoldase activity confers a unique level of substrate specificity to the universal AAA protease FtsH. PubMed: Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. PubMed: Identification and properties of type I-signal peptidases of Bacillus amyloliquefaciens. PubMed: Conformations of proteins in equilibrium. PubMed: ATP-dependent proteases degrade their substrates by processively unraveling them from the degradation signal. PubMed: Proteolysis of mesophilic and thermophilic alpha-amylases: a comparative study. PubMed: Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria. PubMed: [Deletion analysis of the structural-functional organization of metalloendopeptidase precursor in B. amyloliquefaciens]. PubMed: Improving the activity of immobilized subtilisin by site-directed attachment through a genetically engineered affinity tag. PubMed: Detergent-independent in vitro activity of a truncated Bacillus signal peptidase. PubMed: Point mutations and sequence variability in proteins: redistributions of preexisting populations. PubMed: A self-consistent knowledge-based approach to protein design. PubMed: Novel folded protein domains generated by combinatorial shuffling of polypeptide segments. PubMed: The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease. PubMed: Predicting the rate enhancement of protein complex formation from the electrostatic energy of interaction. PubMed: Proteolytic selection for protein folding using filamentous bacteriophages. PubMed: Reasoning enantioselectivity and kinetics of seleno-subtilisin from the subtilisin template. PubMed: Heterogeneity of packing: structural approach. PubMed: Bacillus subtilis contains four closely related type I signal peptidases with overlapping substrate specificities. Constitutive and temporally controlled expression of different sip genes. PubMed: Secretion of authentic 20-kDa human growth hormone (20K hGH) in Escherichia coli and properties of the purified product. PubMed: Modeling unfolded states of proteins and peptides. II. Backbone solvent accessibility. PubMed: Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors. PubMed: Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I. PubMed: Structural characterization of extracellular ribonuclease of Bacillus polymyxa: amino acid sequence determination and spatial structure prediction. PubMed: Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity. PubMed: Structure of the transition state for folding of the 129 aa protein CheY resembles that of a smaller protein, CI-2. PubMed: Bacillus amyloliquefaciens possesses a second type I signal peptidase with extensive sequence similarity to other Bacillus SPases. PubMed: Probing enzymic transition state hydrophobicities. PubMed: Negative activation enthalpies in the kinetics of protein folding. PubMed: [Characteristics of hybrid genes coding functionally-active secretory metalloproteinases from bacilli]. PubMed: [Primary structure of the intracellular serine proteinase from Bacillus amyloliquefaciens. III. Amino acid sequence of peptides obtained by hydrolysis with a Glu,Asp-specific proteinase. Reconstruction of the entire amino acid sequence of the proteinase]. PubMed: Single versus parallel pathways of protein folding and fractional formation of structure in the transition state. PubMed: Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis. PubMed: Protein-protein recognition: crystal structural analysis of a barnase-barstar complex at 2.0-A resolution. PubMed: Sequence of the gene encoding an alkaline serine protease of thermophilic Bacillus smithii. PubMed: Prediction of pH-dependent properties of proteins. PubMed: Escherichia coli signal peptides direct inefficient secretion of an outer membrane protein (OmpA) and periplasmic proteins (maltose-binding protein, ribose-binding protein, and alkaline phosphatase) in Bacillus subtilis. PubMed: [Primary structure of an intracellular serine proteinase from Bacillus amyloliquefaciens. II. Amino acid sequence of peptides in a chymotrypsin hydrolysate]. PubMed: Recognition between a bacterial ribonuclease, barnase, and its natural inhibitor, barstar. PubMed: [Primary structure and catalytic properties of extracellular ribonuclease from Bacillus circulans]. PubMed: Folding of subtilisin BPN': role of the pro-sequence. PubMed: [Metalloproteinase of Bacillus mesentericus, strain V-313]. PubMed: Long-range surface charge-charge interactions in proteins. Comparison of experimental results with calculations from a theoretical method. PubMed: Folding of subtilisin BPN': characterization of a folding intermediate. PubMed: Identification of glutamic acid 105 at the active site of Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan 4-glucanohydrolase using epoxide-based inhibitors. PubMed: Improving the production of E. coli beta-lactamase in Bacillus subtilis: the effect of glucose, pH and temperature on the production level. PubMed: Secretory expression of a glutamic-acid-specific endopeptidase (SPase) from Staphylococcus aureus ATCC12600 in Bacillus subtilis. PubMed: Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching. PubMed: [Design of hybrid metalloproteinases from Bacillus]. PubMed: [Isolation and analysis of protease-deficient mutants of Bacillus amyloliquefaciens]. PubMed: Modular expression and secretion vectors for Bacillus subtilis. PubMed: Secretion of correctly processed and folded pancreatic secretory trypsin inhibitor by Bacillus subtilis. PubMed: Processing of the prepropeptide portions of the Bacillus amyloliquefaciens neutral protease fused to Bacillus subtilis alpha-amylase and human growth hormone during secretion in Bacillus subtilis. PubMed: [Cloning and gene expression of Bacillus cereus neutral proteinase in Bacillus subtilis cells]. PubMed: Expression of Bacillus amyloliquefaciens amylase and Vibrio alginolyticus protease A fusion genes. PubMed: Sequence regions of Bacilli metalloproteinases that can affect enzyme thermostability. PubMed: Cloning and expression of a novel protease gene encoding an extracellular neutral protease from Bacillus subtilis. PubMed: Genetic manipulation of Bacillus amyloliquefaciens. PubMed: Optimization of the signal-sequence cleavage site for secretion from Bacillus subtilis of a 34-amino acid fragment of human parathyroid hormone. PubMed: Use of alkaline phosphatase fusions to study protein secretion in Bacillus subtilis. PubMed: Accurate measurements of coupling constants from two-dimensional nuclear magnetic resonance spectra of proteins and determination of phi-angles. PubMed: [Structure of the Bacillus brevis metalloprotease gene]. PubMed: Bacillus amyloliquefaciens alpha-amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillus subtilis cells. PubMed: Secretion of the alpha-galactosidase from Cyamopsis tetragonoloba (guar) by Bacillus subtilis. PubMed: Protein engineering of disulfide bonds in subtilisin BPN'. PubMed: Expression of bovine pancreatic ribonuclease A coded by a synthetic gene in Bacillus subtilis. PubMed: The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation. PubMed: [Primary structure of intracellular serine proteinase from Bacillus amyloliquefaciens. I. Isolation of the enzyme and amino acid sequence of peptides of tryptic hydrolysate]. PubMed: Dissecting the catalytic triad of a serine protease. PubMed: Engineering thermostability in subtilisin BPN' by in vitro mutagenesis. PubMed: Prediction of electrostatic effects of engineering of protein charges. PubMed: Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure. PubMed: Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering. PubMed: Engineering enzyme specificity by "substrate-assisted catalysis". PubMed: Secretion of human serum albumin from Bacillus subtilis. PubMed: Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double-stranded DNA. PubMed: Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering. PubMed: Proteases of enhanced stability: characterization of a thermostable variant of subtilisin. PubMed: Site-directed mutagenesis and the role of the oxyanion hole in subtilisin. PubMed: In vivo formation and stability of engineered disulfide bonds in subtilisin. PubMed: Secretion and autoproteolytic maturation of subtilisin. PubMed: Tunicamycin-resistant mutants of Bacillus amyloliquefaciens are deficient in amylase, protease and penicillinase synthesis and have altered sensitivity to antibiotics and autolysis. PubMed: Secretion of a heterologous protein from Bacillus subtilis with the aid of protease signal sequences. PubMed: Secretion of Semliki Forest virus membrane glycoprotein E1 from Bacillus subtilis. PubMed: Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. PubMed: Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation. PubMed: Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. PubMed: The characteristics of liver glucose-6-phosphatase in the envelope of isolated nuclei and microsomes are identical. PubMed: [Specific features of intracellular serine proteinase of Bacillus amyloliquefaciens on native and denatured protein substrates]. PubMed: [Bacillus subtilis var. amyloliquefaciens biosynthesis of extracellular protease possessing coagulase activity and formed under conditions of limiting the nitrogen sources in the medium]. PubMed: [Immunologic comparison of intracellular and extracellular serine proteases from Bacillus amyloliquefaciens and some other proteases]. PubMed: The specificity of the Bacillus amyloliquefaciens intracellular serine protease: a comparison with the specificity of secretory subtilisins. PubMed: Sequence of the N-terminal half of Bacillus amyloliquefaciens alpha-amylase. PubMed: Modulation of an apparent mRNA pool for extracellular protease in Bacillus amyloliquefaciens. PubMed: Effect of growth temperature on membrane fatty acid composition and susceptibility to cold shock of Bacillus amyloliquefaciens. PubMed: [Exoprotease properties of Bacillus subtilis var. amyloliquefaciens capable of coagulating blood plasma]. PubMed: Purification and characterization of L-pyrrolidonecarboxylate peptidase from Bacillus amyloiliquefaciens. PubMed: [Effect of different carbon, nitrogen and phosphorus sources on the biosynthesis of proteases with coagulase activity by Bacillus subtilis var, amyloliquefaciens]. PubMed: Evidence for extrusion of unfolded extracellular enzyme polypeptide chains through membranes of Bacillus amyloliquefaciens. PubMed: Release of extracellular enzymes from Bacillus amyloliquefaciens. PubMed: Accumulation of messenger ribonucleic acid specific for extracellular protease in Bacillus subtilis 168. PubMed: Dynamic state of the messenger RNA pool specific for extracellular protease in Bacillus amyloliquefaciens: its relevance to the mechanism of enzyme secretion. PubMed: Amino-acid sequence of extracellular ribonuclease (barnase) of Bacillus amyloliquefaciens. PubMed: Insensitivity of Bacillus amyloliquefaciens extracellular protease formation to rifampicin and actinomycin D.