monellin

monellin PubMed: Picosecond fluorescence dynamics of tryptophan and 5-fluorotryptophan in monellin: slow water-protein relaxation unmasked. PubMed: Improving Flavour and Quality of Tomatoes by Expression of Synthetic Gene Encoding Sweet Protein Monellin. PubMed: Design of sweet protein based sweeteners: hints from structure-function relationships. PubMed: Expression of monellin in a food-grade delivery system in Saccharomyces cerevisiae. PubMed: High-energy intermediates in protein unfolding characterized by thiol labeling under nativelike conditions. PubMed: Amyloid fibril formation by the chain B subunit of monellin occurs by a nucleation-dependent polymerization mechanism. PubMed: Charge dependent retardation of amyloid β aggregation by hydrophilic proteins. PubMed: The utilization of competing unfolding pathways of monellin is dictated by enthalpic barriers. PubMed: A buried ionizable residue destabilizes the native state and the transition state in the folding of monellin. PubMed: Functional characterization of the heterodimeric sweet taste receptor T1R2 and T1R3 from a New World monkey species (squirrel monkey) and its response to sweet-tasting proteins. PubMed: Kinetic studies of the folding of heterodimeric monellin: evidence for switching between alternative parallel pathways. PubMed: The effect of nanoparticles on amyloid aggregation depends on the protein stability and intrinsic aggregation rate. PubMed: Identification of novel sweet protein for nutritional applications. PubMed: Determinants of sweetness in proteins: a topological approach. PubMed: Identification of multiple folding pathways of monellin using pulsed thiol labeling and mass spectrometry. PubMed: Equilibrium unfolding studies of monellin: the double-chain variant appears to be more stable than the single-chain variant. PubMed: Reduced sweetness of a monellin (MNEI) mutant results from increased protein flexibility and disruption of a distant poly-(L-proline) II helix. PubMed: Expression and secretion of a single-chain sweet protein, monellin, in Saccharomyces cerevisiae by an α-factor signal peptide. PubMed: Interactions in the native state of monellin, which play a protective role against aggregation. PubMed: Heterologous expression, purification and characterization of heterodimeric monellin. PubMed: Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor. PubMed: Aggregation mechanisms of cystatins: a comparative study of monellin and oryzacystatin. PubMed: Modeling the fluorescence of protein-embedded tryptophans with ab initio multiconfigurational quantum chemistry: the limiting cases of parvalbumin and monellin. PubMed: Direct evidence for a dry molten globule intermediate during the unfolding of a small protein. PubMed: Continuous dissolution of structure during the unfolding of a small protein. PubMed: GroEL can unfold late intermediates populated on the folding pathways of monellin. PubMed: Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins. PubMed: Role of protein surface charge in monellin sweetness. PubMed: [Cloning, characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92]. PubMed: Dehydration of main-chain amides in the final folding step of single-chain monellin revealed by time-resolved infrared spectroscopy. PubMed: Characterization of the folding and unfolding reactions of single-chain monellin: evidence for multiple intermediates and competing pathways. PubMed: Monellin (MNEI) at 1.15 A resolution. PubMed: Developments in biotechnological production of sweet proteins. PubMed: Riboflavin-binding protein exhibits selective sweet suppression toward protein sweeteners. PubMed: Expression and secretion of a single-chain sweet protein monellin in Bacillus subtilis by sacB promoter and signal peptide. PubMed: Sucrose substitutes and their role in caries prevention. PubMed: The importance of electrostatic potential in the interaction of sweet proteins with the sweet taste receptor. PubMed: Intra- versus intermolecular interactions in monellin: contribution of surface charges to protein assembly. PubMed: Toward the understanding of MNEI sweetness from hydration map surfaces. PubMed: Ultrafast fluorescence dynamics of tryptophan in the proteins monellin and IIAGlc. PubMed: High-level expression of a synthetic gene encoding a sweet protein, monellin, in Escherichia coli. PubMed: [Overexpression of a sweet protein monellin in Escherichia coli]. PubMed: Probing the sweet determinants of brazzein: wild-type brazzein and a tasteless variant, brazzein-ins(R18a-I18b), exhibit different pH-dependent NMR chemical shifts. PubMed: Specific collapse followed by slow hydrogen-bond formation of beta-sheet in the folding of single-chain monellin. PubMed: Sweet proteins--potential replacement for artificial low calorie sweeteners. PubMed: Multi-method global analysis of thermodynamics and kinetics in reconstitution of monellin. PubMed: Structure of monellin refined to 2.3 a resolution in the orthorhombic crystal form. PubMed: The cysteine-rich region of T1R3 determines responses to intensely sweet proteins. PubMed: Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatin. PubMed: Fluorescence studies of protein thermostability in ionic liquids. PubMed: Behavioral study in the gray mouse lemur (Microcebus murinus) using compounds considered sweet by humans. PubMed: Monkey electrophysiological and human psychophysical responses to mutants of the sweet protein brazzein: delineating brazzein sweetness. PubMed: Activation mechanism of HSP16.5 from Methanococcus jannaschii. PubMed: On the mechanism of chaperone activity of the small heat-shock protein of Methanococcus jannaschii. PubMed: The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI. PubMed: Ultrafast surface hydration dynamics and expression of protein functionality: alpha -Chymotrypsin. PubMed: Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor. PubMed: Hydration at the surface of the protein Monellin: dynamics with femtosecond resolution. PubMed: Sense of taste in a new world monkey, the common marmoset: recordings from the chorda tympani and glossopharyngeal nerves. PubMed: Multistep nucleus formation and a separate subunit contribution of the amyloidgenesis of heat-denatured monellin. PubMed: Folding and stability of sweet protein single-chain monellin. An insight to protein engineering. PubMed: Probing the surface of a sweet protein: NMR study of MNEI with a paramagnetic probe. PubMed: Physicochemical evaluation of protein folds predicted by threading. PubMed: Solution structure, backbone dynamics, and stability of a double mutant single-chain monellin. structural origin of sweetness. PubMed: Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin. PubMed: Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica. PubMed: Electrophoresis of proteins and protein-protein complexes in a native agarose gel. PubMed: Recent developments in the characterization and biotechnological production of sweet-tasting proteins. PubMed: Amyloid-like aggregates of a plant protein: a case of a sweet-tasting protein, monellin. PubMed: Enzymatic ligation for synthesis of single-chain analogue of monellin by transglutaminase. PubMed: Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations. PubMed: Structural differences in D and L-monellin in the crystals of racemic mixture. PubMed: Structure of an enantiomeric protein, D-monellin at 1.8 A resolution. PubMed: Solid-phase synthesis of single-chain monellin, a sweet protein. PubMed: Small heat shock protein of Methanococcus jannaschii, a hyperthermophile. PubMed: Solution structure of the thermostable sweet-tasting protein brazzein. PubMed: Measurement of Fluorescence from Tryptophan To Probe the Environment and Reaction Kinetics within Protein-Doped Sol-Gel-Derived Glass Monoliths. PubMed: Structure and dynamic studies by NMR of the potent sweet protein monellin and a non-sweet analog. Evidence on the importance of residue AspB7 for sweet taste. PubMed: Taste in chimpanzees II: single chorda tympani fibers. PubMed: Crystallization and preliminary X-ray analysis of D-monellin. PubMed: High-level expression of a sweet protein, monellin, in the food yeast Candida utilis. PubMed: Sweet science: overexpression of monellin in yeast. PubMed: Gustatory receptor cell responses to the sweeteners, monellin and thaumatin. PubMed: Possible active site of the sweet-tasting protein thaumatin. PubMed: Detection of water proximity to tryptophan residues in proteins by single photon radioluminescence. PubMed: Controlling susceptibility against protease digestion. PubMed: The taste-active regions of monellin, a potently sweet protein. PubMed: Production and characterization of cross-reactive monoclonal antibodies to sweet proteins. PubMed: Two crystal structures of a potently sweet protein. Natural monellin at 2.75 A resolution and single-chain monellin at 1.7 A resolution. PubMed: Monoclonal antibodies to sweet taste proteins: II. Development of two different immunoassays for thaumatin and monellin. PubMed: Sweet-tasting protein monellin is related to the cystatin family of thiol proteinase inhibitors. PubMed: Structural characterization of monellin in the alcohol-denatured state by NMR: evidence for beta-sheet to alpha-helix conversion. PubMed: Gustatory neural coding in the monkey cortex: the quality of sweetness. PubMed: Highly probable active site of the sweet protein monellin. PubMed: 1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by 1H 2D-NMR. PubMed: Structural determination of the active site of a sweet protein. A 1H NMR investigation of pMNEI. PubMed: Solid-phase synthesis of [AsnA16]-, [AsnA22]-, [GlnA25]-, and [AsnA26]monellin, analogues of the sweet protein monellin. PubMed: An enzyme immunoassay and immunoblot analysis for curculin, a new type of taste-modifying protein: cross-reactivity of curculin and miraculin to both antibodies. PubMed: Characteristics of antisweet substances, sweet proteins, and sweetness-inducing proteins. PubMed: Monoclonal antibodies to sweet taste proteins. I. Analysis of antigenic epitopes on thaumatin by competitive inhibition assays. PubMed: Solid-phase synthesis of crystalline [Ser41] B-chain monellin, an analogue of the sweet protein monellin. PubMed: Solid-phase synthesis of crystalline monellin, a sweet protein. PubMed: Anisotropy decays of single tryptophan proteins measured by GHz frequency-domain fluorometry with collisional quenching. PubMed: Solid-phase synthesis and crystallization of [Asn22, Gln25, Asn26]-A-chain-[Asn49, Glu50]-B-chain-monellin, an analogue of the sweet protein monellin. PubMed: The locked rotation function. PubMed: Purification and complete amino acid sequence of a new type of sweet protein taste-modifying activity, curculin. PubMed: Complete amino acid sequence of the sweet protein monellin. PubMed: Solid-phase synthesis and crystallization of monellin, an intensely sweet protein. PubMed: Redesigning a sweet protein: increased stability and renaturability. PubMed: The sweet taste in the calf. II. Glossopharyngeal nerve responses to taste stimulation of the tongue. PubMed: Three-dimensional reconstructions from incomplete data: interpretability of density maps at "atomic" resolution. PubMed: The sweet taste in the calf. I. Chorda tympani proper nerve responses to taste stimulation of the tongue. PubMed: Binding proteins for sweet compounds from gustatory papillae of the cow, pig and rat. PubMed: Crystal structures of two intensely sweet proteins. PubMed: Does the fluorescence quencher acrylamide bind to proteins? PubMed: Crystal structure of the intensely sweet protein monellin. PubMed: The association of acrylamide with proteins. The interpretation of fluorescence quenching experiments. PubMed: Measurement of subnanosecond anisotropy decays of protein fluorescence using frequency-domain fluorometry. PubMed: Effects of gymnemic acid on the chorda tympani proper nerve responses to sweet, sour, salty and bitter taste stimuli in the chimpanzee. PubMed: Conformational changes of the sweet protein monellin as measured by fluorescence emission. PubMed: Three-dimensional structure of thaumatin I, an intensely sweet protein. PubMed: Peptide interactions with taste receptors: overlap in taste receptor specificity. PubMed: Fast atom bombardment - high field magnet mass spectrometry of 6000 dalton polypeptides. PubMed: Preliminary crystallographic studies of a sweet protein, monellin. PubMed: Fluorescence quenching studies with proteins. PubMed: Formation of oligomeric monellin in protein denaturants. PubMed: Enzymatic properties of the sweet-tasting proteins thaumatin and monellin after partial reduction. PubMed: Non-nutritive sweetening agents. PubMed: Monellin, a sweet-tasting protein, and the biochemistry of sweet taste. PubMed: Qualitative differences among sweeteners. PubMed: The complete amino-acid sequence of the sweet protein thaumatin I. PubMed: Effect of the sweet-tasting protein monellin on growth of a cariogenic bacterium: a microbiological study. PubMed: Biochemical studies of taste sensation: binding to taste tissue of 3H-labeled monellin, a sweet-tasting protein. PubMed: Methylation of the lysine residues of monellin. PubMed: The taste responses in primates to the proteins thaumatin and monellin and their phylogenetic implications. PubMed: Fluorescence characteristics of native and denatured monellin. PubMed: Conformational transitions of monellin, an intensely sweet protein. PubMed: The sulfhydryl group of monellin: its chemical reactivity and importance to the sweet taste. PubMed: Enzymatic proteolysis of monellin. Absence of sweet peptides. PubMed: Mass spectrometric sequencing of proteins. The structure of subunit I of monellin. PubMed: Gustatory effects of miraculin, monellin and thaumatin in the Saguinus midas tamarin monkey studied with electrophysiological and behavioural techniques. PubMed: The complete amino acid sequences of both subunits of the sweet protein monellin. PubMed: The structure of monellin and its relation to the sweetness of the protein. PubMed: Sweetening agents from natural sources. PubMed: Proceedings: Immunological studies with monellin, a protein that tastes sweet. PubMed: Effects of denaturants on the sweet-tasting protein monellin. PubMed: Crystallization and crystal data of monellin. PubMed: Electrophysiological study of the gustatory effects of the sweet proteins monellin and thaumatin in monkey, guinea pig and rat. PubMed: Chemostimulatory protein: a new type of taste stimulus. PubMed: Characterization of monellin, a protein that tastes sweet. PubMed: Purification of monellin, the sweet principle of Dioscoreophyllum cumminsii.