lipase from rhizopus oryzae

lipase enzyme preparation from rhizopus oryzae PubMed: Biocatalytic behaviour of immobilized Rhizopus oryzae lipase in the 1,3-selective ethanolysis of sunflower oil to obtain a biofuel similar to biodiesel. PubMed: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris. PubMed: Monoolein production by triglycerides hydrolysis using immobilized Rhizopus oryzae lipase. PubMed: Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei. PubMed: Eco-friendly methodology for efficient synthesis and scale-up of 2-ethylhexyl-p-methoxycinnamate using Rhizopus oryzae lipase and its biological evaluation. PubMed: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase. PubMed: Study of different reaction schemes for the enzymatic synthesis of polyglycerol polyricinoleate. PubMed: High-level expression of pro-form lipase from Rhizopus oryzae in Pichia pastoris and its purification and characterization. PubMed: Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology. PubMed: Organic synthesis of maize starch-based polymer using Rhizopus oryzae lipase, scale up, and its characterization. PubMed: Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres. PubMed: Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived (13)C-labelling data from proteinogenic amino acids. PubMed: Solvent-Free Synthesis of Flavour Esters through Immobilized Lipase Mediated Transesterification. PubMed: Lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films. PubMed: Role of Met93 and Thr96 in the lid hinge region of Rhizopus chinensis lipase. PubMed: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production. PubMed: Assessment of activities and conformation of lipases treated with sub- and supercritical carbon dioxide. PubMed: Esterification of polyglycerol with polycondensed ricinoleic acid catalysed by immobilised Rhizopus oryzae lipase. PubMed: Enzymatic transesterification of palm stearin and olein blends to produce zero-trans margarine fat. PubMed: Synthesis of 2-monoacylglycerols and structured triacylglycerols rich in polyunsaturated fatty acids by enzyme catalyzed reactions. PubMed: Immobilization of lipase from Mucor miehei and Rhizopus oryzae into mesoporous silica--the effect of varied particle size and morphology. PubMed: Crosslinked aggregates of Rhizopus oryzae lipase as industrial biocatalysts: preparation, optimization, characterization, and application for enantioselective resolution reactions. PubMed: Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment V(HH) against EGFR. PubMed: A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichia. PubMed: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures. PubMed: Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil). PubMed: Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris. PubMed: Heterologous expression systems for lipases: a review. PubMed: Characterization of smallest active monomeric lipase from novel rhizopus strain: application in transesterification. PubMed: Production of recombinant Rhizopus oryzae lipase by the yeast Yarrowia lipolytica results in increased enzymatic thermostability. PubMed: Water activity dependence of performance of surface-displayed lipase in yeast cells: a unique water requirement for enzymatic synthetic reaction in organic media. PubMed: Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide. PubMed: Expression and characterization of recombinant Rhizopus oryzae lipase for enzymatic biodiesel production. PubMed: Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity. PubMed: Fungi isolated from olive ecosystems and screening of their potential biotechnological use. PubMed: Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: optimization by response surface methodology. PubMed: Immobilization and stability of a Rhizopus oryzae lipase expressed in Pichia pastoris: comparison between native and recombinant variants. PubMed: Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst. PubMed: Mechanism of acetaldehyde-induced deactivation of microbial lipases. PubMed: Isolation, identification and optimization of a new extracellular lipase producing strain of Rhizopus sp. PubMed: A large scale enzyme screen in the search for new methods of silicon-oxygen bond formation. PubMed: Enantioselective production of benzoin from benzoin acetate via kinetic resolution and deracemization using Rhizopus oryzae. PubMed: Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. PubMed: Enzyme mediated silicon-oxygen bond formation; the use of Rhizopus oryzae lipase, lysozyme and phytase under mild conditions. PubMed: Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media. PubMed: Strategies for utilisation of food-processing wastes to produce lipases in solid-state cultures of Rhizopus oryzae. PubMed: Structural homologies, importance for catalysis and lipid binding of the N-terminal peptide of a fungal and a pancreatic lipase. PubMed: Significant changes in the transesterification activity of free and mesoporous-immobilized Rhizopus oryzae lipase in ionic liquids. PubMed: [Stability of whole cell biocatalyst for biodiesel production from renewable oils]. PubMed: Preparation of (S)-1-Halo-2-octanols Using Ionic Liquids and Biocatalysts. PubMed: Development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized Candida rugosa and Rhizopus oryzae lipases. PubMed: Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports. PubMed: Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter. PubMed: Water activity dependence of lipases in non-aqueous biocatalysis. PubMed: Effect of a buffer mixture system on the activity of lipases during immobilization process. PubMed: Screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest. PubMed: Optimization of the process for biodiesel production using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. PubMed: Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. PubMed: Physical immobilization of Rhizopus oryzae lipase onto cellulose substrate: activity and stability studies. PubMed: Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae. PubMed: Optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity. PubMed: Enhancement of the stability of a prolipase from Rhizopus oryzae toward aldehydes by saturation mutagenesis. PubMed: Comparative study of thermostability and ester synthesis ability of free and immobilized lipases on cross linked silica gel. PubMed: Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. PubMed: Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies. PubMed: Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris. PubMed: Production of butyl acetate ester by lipase from novel strain of Rhizopus oryzae. PubMed: Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. PubMed: Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. PubMed: Preparation of a whole-cell biocatalyst of mutated Candida antarctica lipase B (mCALB) by a yeast molecular display system and its practical properties. PubMed: Combining regio- and enantioselectivity of lipases for the preparation of (R)-4-chloro-2-butanol. PubMed: Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris. PubMed: Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate. PubMed: Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system. PubMed: Biochemical and molecular characterization of a lipase produced by Rhizopus oryzae. PubMed: Plasmids for expression of heterologous proteins in Rhizopus oryzae. PubMed: A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess. PubMed: Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. PubMed: Lipase-catalyzed esterification of 2-monoricinolein for 1,2 (2,3)-diricinolein synthesis. PubMed: State variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by Pichia pastoris. PubMed: Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli. PubMed: Olive-mill wastewaters: a promising substrate for microbial lipase production. PubMed: Cell surface engineering of yeast: construction of arming yeast with biocatalyst. PubMed: Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent. PubMed: Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents. PubMed: Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter. PubMed: Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. PubMed: Creation of Rhizopus oryzae lipase having a unique oxyanion hole by combinatorial mutagenesis in the lid domain. PubMed: N-terminal peptide of Rhizopus oryzae lipase is important for its catalytic properties. PubMed: Model studies on the formation of monochloropropanediols in the presence of lipase. PubMed: Use of immobilized lipases for lipase purification via specific lipase-lipase interactions. PubMed: Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. PubMed: Enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. PubMed: Fluorescent organophosphonates as inhibitors of microbial lipases. PubMed: Evaluation of the catalytic activity of lipases immobilized on chrysotile for esterification. PubMed: Endophytic fungi associated with Mediterranean plants as a source of mycelium-bound lipases. PubMed: Safety evaluation of lipase produced from Rhizopus oryzae: summary of toxicological data. PubMed: Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. PubMed: Gas-phase enzymatic esterification on immobilized lipases in MCM-41 molecular sieves. PubMed: Fluorescent inhibitors reveal solvent-dependent micropolarity in the lipid binding sites of lipases. PubMed: Synthesis of phosphatidylcholine with defined fatty acid in the sn-1 position by lipase-catalyzed esterification and transesterification reaction. PubMed: Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production. PubMed: Lipase-catalysed hydrolysis of short-chain substrates in solution and in emulsion: a kinetic study. PubMed: Spacer-mediated display of active lipase on the yeast cell surface. PubMed: Kinetic studies of Rhizopus oryzae lipase using monomolecular film technique. PubMed: Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae. PubMed: Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles. PubMed: Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. PubMed: The His gap motif in microbial lipases: a determinant of stereoselectivity toward triacylglycerols and analogs. PubMed: Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. PubMed: Independent production of two molecular forms of a recombinant Rhizopus oryzae lipase by KEX2-engineered strains of Saccharomyces cerevisiae. PubMed: Molecular dynamics of microbial lipases as determined from their intrinsic tryptophan fluorescence. PubMed: Interactions of fluorescent triacylglycerol analogs covalently bound to the active site of a lipase from Rhizopus oryzae. PubMed: A toolbox of recombinant lipases for industrial applications. PubMed: Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. PubMed: Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. PubMed: Rational design of Rhizopus oryzae lipase with modified stereoselectivity toward triradylglycerols. PubMed: Inhibition of lipases from Chromobacterium viscosum and Rhizopus oryzae by tetrahydrolipstatin. PubMed: Safety evaluation of lipase derived from Rhizopus oryzae: summary of toxicological data. PubMed: Inhibition of microbial lipases with stereoisomeric triradylglycerol analog phosphonates. PubMed: The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. PubMed: Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants. PubMed: Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae. PubMed: [Premedication for sonography. Enzyme-paractol in comparison with a placebo]. PubMed: [Immobilized lipase from Rhizopus oryzae 14-14]. PubMed: [Experiences with substitution therapy using a new pancreatic enzyme of plant origin].